2024

High resolution live cell imaging to define ultrastructural and dynamic features of the halotolerant yeast Debaryomyces hansenii
Biology Open
Xelhuantzi MSC, Ghete D, Milburn A, Ioannou S, Mudd P, Calder G, Ramos J, O’Toole PJ, Genever PG, MacDonald C.

2023

The phosphatase Glc7 controls eisosomal response to starvation via posttranslational modification of Pil1
Journal of Cell Science
Paine KM, Laidlaw KM, Evans GJO, MacDonald C.

Discovery of a rapidly evolving yeast defense factor, KTD1, against the secreted killer toxin K28
PNAS
Andreev I, Laidlaw KM, Giovanetti SM, Urtecho G, Shriner D, Bloom JS, MacDonald C, Sadhu MJ.


2022

Ubiquitination of Ist1 controls surface recycling from endosomes
Journal of Cell Biology
Laidlaw KM, Calder G, MacDonald C.

Correlating viscosity and molecular crowding with fluorescent nanobeads and molecular probes: in vitro and in vivo
Interface Focus 
Lecinski S*, Shepherd JW*, Bunting K, Dresser L, Quinn S, MacDonald C, Leake MC.

The effect of lithium on the budding yeast Saccharomyces cerevisiae upon stress adaptation
Microorganisms 
Reith P, Braam S, Welkenhuysen N, Lecinski S, Shepherd J, MacDonald C, Leake MC, Hohmann S,
Shashkova S, Cvijovic M.

Endosomal cargo recycling mediated by Gpa1 and phosphatidylinositol 3-kinase is inhibited by glucose starvation
Molecular Biology of the Cell
Laidlaw KM*, Paine KM*, Bisinski DD, Calder G, Hogg K, Ahmed S, James S, O’Toole PJ, MacDonald C.


2021

The Rpd3-complex regulates expression of multiple cell surface recycling factors in yeast
International Journal of Molecular Science
Amoiradaki K, Bunting KR, Paine KM, Ayre JE, Hogg K, Laidlaw KM, MacDonald C.

Investigating molecular crowding during cell division and hyperosmotic stress in budding yeast with FRET
Current Topics in Membranes
Lecinski S*, Shepherd JW*, Frame L, Hayton I, MacDonald C, Leake MC.

Fur4 mediated uracil-scavenging to screen for surface protein regulators
Traffic
Paine KM, Ecclestone G, MacDonald C. 

A glucose-starvation response governs endocytic trafficking and eisosomal retention surface cargoes in budding yeast
Journal of Cell Science
Laidlaw K, Bisinski D, Shashkova S, Paine K, Veillon M, Leake M, MacDonald C. 


2020

Molecular crowding in single eukaryotic cells: using cell environment biosensing and single-molecule optical microscopy to probe dependence on extracellular ionic strength, local glucose conditions, and sensor copy number
Methods
Shepherd JW, Lecinski S, Wragg J, Shashkova S, MacDonald C, Leake MC.

A Cycle of Ubiquitination Regulates Adaptor Function of the Nedd4-Family Ubiquitin Ligase Rsp5
Current Biology
MacDonald C, Shields SB, Williams CA, Winistorfer SC, Piper RC.


2018

Endosomal trafficking of yeast membrane proteins
Biochemical Society Transactions
Laidlaw K & MacDonald C.


2017

COPI mediates recycling of an exocytic SNARE by recognition of a ubiquitin sorting signal
eLife
Xu P, Hankins H, MacDonald C, Erlinger S, Frazier M, Diab N, Piper RC, Jackson L, MacGurn J, Graham T. 

Genetic dissection of early endosomal recycling highlights a TORC1-independent role for Rag GTPases
Journal of Cell Biology
MacDonald C & Piper RC. 

Enzyme reversal to explore the function of yeast E3 ubiquitin-ligases
Traffic
MacDonald C, Winistorfer SC, Pope M, Wight ME, Piper RC.


2016

Cell surface recycling in yeast: mechanisms and machineries
Biochemical Society Transactions
MacDonald C & Piper RC. 


2015

Tetraspan cargo adaptors usher GPI-anchored proteins into multivesicular bodies
Cell Cycle
MacDonald C, Stamnes MA, Katzmann, DJ & Piper RC. 

Puromycin and methotrexate resistance cassettes and optimized Cre-recombinase expression plasmids for use in yeast
Yeast
MacDonald C & Piper RC. 

A Family of Tetraspans Organizes Cargo for Sorting into Multivesicular Bodies
Developmental Cell
MacDonald C, Payne, JA, Aboian, M, Smith W, Katzmann, DJ & Piper RC. 


2013

Mechanism of ubiquitin ligation and lysine prioritization by a HECT E3
eLife
Kamadurai HB, Qiu Y, Deng A, Harrison JS, MacDonald C, Actis M, Rodrigues P, Miller DJ, Souphron J, Lewis SM, Kurinov I, Fujii N, Hammel M, Piper R, Kuhlman B, Schulman BA.


2012

Cargo ubiquitination is essential for multivesicular body intralumenal vesicle formation
EMBO Reports
MacDonald C, Buchkovich NJ, Stringer DK, Emr SD, Piper RC. 

Sna3 Is an Rsp5 Adaptor Protein that Relies on Ubiquitination for Its MVB Sorting
Traffic
MacDonald C, Stringer DK, Piper RC. 


2010

Autoinhibition of SNARE complex assembly by a conformational switch represents a conserved feature of syntaxins
Biochemical Society Transactions
MacDonald C, Munson M, Bryant NJ. 


2009

The N-terminal peptide of the syntaxin Tlg2p modulates binding of its closed conformation to Vps45p
Proceedings of the National Academy of Sciences
Furgason ML, MacDonald C, Shanks SG, Ryder SP, Bryant NJ, Munson M.

Functional homology of mammalian syntaxin 16 and yeast Tlg2p reveals a conserved regulatory mechanism
Journal of Cell Science
Struthers MS, Shanks SG, MacDonald C, Carpp LN, Drozdowska AM, Kioumourtzoglou D, Furgason ML, Munson M, Bryant NJ.